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Cas9 Nuclease (10mg/mL) - 500 µg

Cas9 Nuclease (10mg/mL) - 500 µg

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Cas9 Nuclease, derived from wild-type Streptococcus pyogenes, is an RNA-guided endonuclease capable of specifically cleaving double-stranded DNA (it can also cleave single-stranded DNA or RNA in the presence of DNA PAM). The Cas9 cleavage site is located within the target sequence, three bases away from the PAM (NGG) region. This product, through codon optimization and nuclear localization signal (NLS) design, is recombinantly expressed in Escherichia coli, exhibiting high editing efficiency.

Cas9 (CRISPR-associated protein 9) is a bacterial endonuclease enzyme that plays a central role in the CRISPR-Cas adaptive immune system found in Streptococcus pyogenes and other bacteria. It is widely used as a genome editing tool in research, biotechnology, and medicine.


Function: Cas9 is an RNA-guided DNA endonuclease that introduces double-strand breaks (DSBs) at specific genomic locations.

Guide RNA (gRNA): Cas9 is directed to its target sequence by a complementary guide RNA, allowing programmable gene editing.

PAM requirement: Cas9 recognizes a protospacer adjacent motif (PAM) (typically “NGG”) near the target site to bind and cleave DNA.

Origin: Naturally found in S. pyogenes but engineered versions (e.g., SpCas9, SaCas9) are widely used in mammalian and plant systems.

123,456.78 € 123456.78 EUR 123,456.78 €

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