HisSep Ni-NTA Agarose Resin - 100 mL
HisSep Ni-NTA Agarose Resin is a high-performance affinity chromatography medium designed for the purification of His-tagged recombinant proteins. The resin consists of highly cross-linked agarose beads functionalized with nickel-charged nitrilotriacetic acid (Ni-NTA), ensuring strong and specific binding to polyhistidine tags under native or denaturing conditions.
Key Features:
✅ High binding capacity for His-tagged proteins
✅ Excellent chemical and physical stability for repeated use
✅ Compatible with both native and denaturing purification protocols
✅ Minimal non-specific binding for greater purity
Applications:
- Purification of His-tagged recombinant proteins from bacterial, yeast, insect, or mammalian expression systems
- Batch or column-based affinity chromatography
- Protein interaction studies
Specifications:
- Matrix: Highly cross-linked agarose
- Ligand: Ni-NTA
- Format: 100 mL resin slurry